Harmonization of antimicrobial susceptibility testing by broth microdilution for Rhodococcus equi of animal origin

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Harmonization of antimicrobial susceptibility testing by broth microdilution for Rhodococcus equi of animal origin.

genes. The PCR-based replicon typing method showed that this blaNDM-1-positive plasmid belonged to the IncHI incompatibility group. Genetic structures surrounding the blaNDM-1 gene were investigated using a PCR mapping approach and sequencing. The blaNDM-1 gene was immediately bracketed by a truncated insertion sequence ISAba125 upstream and the bleomycin resistance gene bleMBL downstream. This...

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Comparison of Etest, disk diffusion, and broth macrodilution for in vitro susceptibility testing of Rhodococcus equi.

MICs of erythromycin, clarithromycin, azithromycin, rifampin, gentamicin, and doxycycline against 101 isolates of Rhodococcus equi were determined by broth macrodilution, disk diffusion, and Etest. Categorical agreement ranged between 85.1 and 100%. Overall, the agreement between Etest and disk diffusion was better than the agreement between broth macrodilution and the agar-based methods.

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Antimicrobial susceptibility testing of Acinetobacter spp. by NCCLS broth microdilution and disk diffusion methods.

Although both broth microdilution (BMD) and disk diffusion (DD) are listed by NCCLS as acceptable methods for testing Acinetobacter spp. for antimicrobial susceptibility, few studies have compared the results generated by the two methods. We tested 196 isolates of Acinetobacter spp. from nine U.S. hospitals and from the Centers for Disease Control culture collection by using BMD and DD and clin...

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Comparison of Etest method with reference broth microdilution method for antimicrobial susceptibility testing of Yersinia pestis.

The utility of Etest for antimicrobial susceptibility testing of Yersinia pestis was evaluated in comparison with broth microdilution and disk diffusion for eight agents. Four laboratories tested 26 diverse strains and found Etest to be reliable for testing antimicrobial agents used to treat Y. pestis, except for chloramphenicol and trimethoprim-sulfamethoxazole. Disk diffusion testing is not r...

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Use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae.

A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth supplemented with 3% lysed horse blood in parallel with HTM. Inocula of 5 X 1...

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ژورنال

عنوان ژورنال: Journal of Antimicrobial Chemotherapy

سال: 2013

ISSN: 1460-2091,0305-7453

DOI: 10.1093/jac/dkt134